Protocol for Disome Profiling to Survey Ribosome Collision in Humans and Zebrafish

Protocol for Disome Profiling to Survey Ribosome Collision in Humans and Zebrafish

Blog Article

Summary: Ribosomes often encounter obstacles during translation elongation and thus collide with each other.Disome COENZYME Q10 200 MG profiling, an optimized ribosome profiling method, specifically sequences the long ribosome footprints generated from collided ribosomes produced by the ribosome pause and thus allows the survey of Western bit sites in a genome-wide manner.This protocol details the procedure from lysate preparation of human tissue cultures and zebrafish embryos to sequencing library construction.

For complete details on the use and execution of this protocol, please refer to Han et al.(2020).